20121017 Lab 7 Continuation of N. crassa genetics

Goals:
1. Continuation of Neurospora genetics 
2. Observation of field collected fungal samples

1. Continuation of Neurospora genetics 
Steps:
1.1. Take out the Neurospora plate where 2 strains crossed.

Successful cross for Neurospora Crassa

Neurospora crassa under dissecting microscope

 1.2. Pick the perithecium by using a dissecting needle put on glass slide with a drop of autoclaved water and then made a crush mount. Observe under the microscope

2. Observation of field collected fungal samples

Morel: 

Morchella genus, Pezizomycetes class, Ascomycota. 

The honey-comb shaped ascocarps are prized by french cuisine. The Morchella has the antioxidant function and can improve the liver function. Also, it is reported that it contain high macronutrient level, like fiber, which provides cardiovascular and weight loss support. Besides, it also contains high vitamin level.

http://www.livestrong.com/article/446035-what-are-the-health-benefits-of-morel-mushrooms/


Stinkhorn mushroom:
Phallaceae, Agaricomycetes class, Basidiomycota
Stinkhorn mushroom is believed to be a smelly but beneficial fungi. It produces a foul, meat-rotting smell to attract the insects to help them with spore spreading. Though, the smell may be putrid, it indeed help the garden with breaking down the mulch and enable the nutrient to be absorbed by the plants.

Reference from wikipedia

Bird's nest fungi:
Nidulariaceae family, Agaricomycotina subphylum, Basidiomycota
The Bird's nest fungi usually resemble the bird's egg-filled-in nest. It is saprobic and usually can be found on the decay wood. The bird's nest fungi are famous for the special mechanism of spore dispersal. The egg shaped structure contains the spores, which are called as peridioles. Dr. Harold Brodie determined the mechanism as force can released the spores and the spores can attach to a substrate, which enable the spores eaten by an animal and transported to everywhere.

Reference from wikipedia

http://www.botany.hawaii.edu/faculty/wong/BOT135/Lect05_c.htm (interesting website describing all kinds of mechanisms of spore dispersal in fungi)