Wednesday, October 10, 2012

Lab 3 20120918


1. Riddell Mount:
Last week, we learned an important mount method: riddell mount.
Microscopic preparations are essential for an accurate identification of an organism. However, fungi that have delicate sporing structures are easily disturbed upon removal.
Compared to squash mount and tape mount, slide culture is a better method, which involves growing the fungus on a plug of agar, which is resting on a coverslip; the coverslip can then be mounted directly onto a microscope slide.
The obvious drawback of this method is the short life span of the fungus because of the limited nutrient source.


 Pic 1 Riddell Mount of Aspergillus flavus

Pic 2 Riddell Mount of Aspergillus niger

2. Phytophtrora infesta 
2.1 Phytophtrora infesta sporangiaphore

Pic 3 Phytophtrora infesta sporangiaphore
2.2 Phytophtrora infesta zoospore


Video Phytophtrora infesta zoospore

From the video, we can see the movement of zoospores. However, I didn't see the clearly about the mophology of the zoospores.

This mount got contamination unfortunately....

2 comments:

  1. any images from the Riddell mounts?

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    Replies
    1. Yeah, I have 2 Riddell mounts. However, I thing it got contamination, so I didn't post. I have edited the blog. Please check it. Thanks!

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